Serum free Cell Cryopreservation Medium（5% DMSO & USP-Grade)
Product Description:
This is a newly developed cryopreservation medium, a chemically defined cell preservation medium. And all raw materials for this cryopreservation solution are USP-grade (US Pharmacopeia compliant). What's more special is that the DMSO of this cryopreservation solution is only 5%.
Application:
Special for a variety of cell lines and primary cells.for a variety of cell lines and primary cells have been tested but not limited to (for reference) 293T, A539, K562, U937, JurKat, SP2/0, peripheral blood mononuclear cells, adipose-derived mesenchymal stem cells, etc.
Features：
- Read- to-use,easy to use and stable in storage
- Chemically defined, (No animal derived,no human derived, no protein)
- No programmed cooling process required
- High cell recovery viability >90% (after resuscitation) or more
- It can store cells in - 80 ℃ or liquid nitrogen for a long-time,safe and stable
Instructions:
1)Collect cells in logarithmic growth phase in culture.
2)Wash the cells once with 1xPBS or the corresponding cell wash solution, and remove the supernatant by centrifugation.
3)Add an appropriate amount of cell cryopreservation solution according to the number of cells, add slowly, gently resuspend the cells, and transfer to a cell cryopreservation tube.
4)Place the cell cryovial in an appropriate freezing container (eg, a cell cryopreservation box containing isopropanol is better for cryopreservation), and place it at 4 degrees or room temperature for 10-15 minutes (this step is for Make sure that the DMSO has enough time to enter the cell)
5)Store in the -80 degree refrigerator.
6)Reminder: If the cells need to be frozen in a liquid nitrogen tank, the cells should be stored in a -80 degree refrigerator for at least 24 hours.
